2O5I
Crystal structure of the T. thermophilus RNA polymerase elongation complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 41 |
| Unit cell lengths | 156.211, 156.211, 499.229 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.2402 |
| Rwork | 0.238 |
| R-free | 0.26670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2be5 |
| RMSD bond length | 0.159 |
| RMSD bond angle | 1.890 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.423 |
| Number of reflections | 368781 | |
| <I/σ(I)> | 7.1 | 2.5 |
| Completeness [%] | 90.2 | 81.2 |
| Redundancy | 2.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 30% MPD, 0.1M Na cacodylate, 0.2M Mg acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | Na cacodylate | ||
| 3 | 1 | 1 | Mg acetate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | Na cacodylate | ||
| 7 | 1 | 2 | Mg acetate | ||
| 8 | 1 | 2 | H2O |
