2O5E
Structure of E. coli topoisomerase III in complex with an 8-base single stranded oligonucleotide. Frozen in glucose pH 7.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-01-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9479 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 102.260, 102.260, 445.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.132 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.060 | 0.328 |
Total number of observations | 14007 | |
Number of reflections | 79675 | |
<I/σ(I)> | 8.7 | 2.3 |
Completeness [%] | 96.5 | 93 |
Redundancy | 4 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 1.5 M (NH4)SO4, 0.1M Sodium citrate, 0.5 NaCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | (NH4)SO4 | ||
2 | 1 | 1 | Sodium citrate | ||
3 | 1 | 1 | NaCl | ||
4 | 1 | 1 | HOH | ||
5 | 1 | 2 | (NH4)SO4 | ||
6 | 1 | 2 | Sodium citrate | ||
7 | 1 | 2 | NaCl | ||
8 | 1 | 2 | HOH |