2O3F
Structural Genomics, the crystal structure of the N-terminal domain of the putative transcriptional regulator ybbH from Bacillus subtilis subsp. subtilis str. 168.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-07 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 58.898, 48.060, 58.854 |
| Unit cell angles | 90.00, 119.92, 90.00 |
Refinement procedure
| Resolution | 25.520 - 1.750 |
| R-factor | 0.18282 |
| Rwork | 0.181 |
| R-free | 0.21452 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.725 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.520 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.069 | 0.478 |
| Number of reflections | 28223 | |
| <I/σ(I)> | 40.35 | 2.42 |
| Completeness [%] | 98.2 | 86.7 |
| Redundancy | 6.7 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1M Sodium acetate, 2M Ammonium sulfate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
