2O39
Human Adenovirus type 11 knob in complex with domains SCR1 and SCR2 of CD46 (membrane cofactor protein, MCP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-10 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 3 |
Unit cell lengths | 106.140, 106.140, 68.320 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.850 |
R-factor | 0.238 |
Rwork | 0.238 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Human Adenovirus type 11 (unpublished) CD46 SCR1 and SCR2 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.138 | 0.325 |
Number of reflections | 18738 | |
<I/σ(I)> | 7.23 | 2.64 |
Completeness [%] | 93.1 | 83 |
Redundancy | 4.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 35% PEG 200, 200mM CaCl2, 100mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |