2O2Y
The crystal structure of P. falciparum enoyl acyl carrier protein reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9600 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 88.184, 82.368, 94.824 |
Unit cell angles | 90.00, 90.77, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.20211 |
Rwork | 0.200 |
R-free | 0.24562 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1d7o |
RMSD bond length | 0.010 |
RMSD bond angle | 1.286 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.095 | 0.395 |
Number of reflections | 77321 | |
Completeness [%] | 97.2 | 94.7 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 19.5% w/v PEG 3350, 200mM KI, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |