2O2P
Crystal structure of the C-terminal domain of rat 10'formyltetrahydrofolate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 259.500, 194.400, 97.300 |
| Unit cell angles | 90.00, 108.90, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.175 |
| Rwork | 0.174 |
| R-free | 0.18900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2o2q |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.256 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.085 | 0.558 |
| Number of reflections | 485421 | |
| <I/σ(I)> | 10.1 | 2.5 |
| Completeness [%] | 97.5 | 95.8 |
| Redundancy | 5.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | 1.4 M AMMONIUM SULPHATE, 0.1M TRIS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
