2O27
Structure of a class III RTK signaling assembly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 |
Unit cell lengths | 43.270, 43.270, 248.433 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.740 - 2.200 |
R-factor | 0.242 |
Rwork | 0.242 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o26 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.100 | 0.490 |
Number of reflections | 13625 | |
<I/σ(I)> | 43.5 | 5 |
Completeness [%] | 98.9 | 100 |
Redundancy | 13 | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.8M ammonium sulfate, 0.1M Hepes, pH pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |