2O27

Structure of a class III RTK signaling assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-15
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	43.270, 43.270, 248.433
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.740 - 2.200
R-factor	0.242
Rwork	0.242
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o26
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.100	0.490
Number of reflections	13625
<I/σ(I)>	43.5	5
Completeness [%]	98.9	100
Redundancy	13	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.8M ammonium sulfate, 0.1M Hepes, pH pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K