2O20
Crystal structure of transcription regulator CcpA of Lactococcus lactis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-12 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 117.756, 74.267, 160.302 |
Unit cell angles | 90.00, 102.36, 90.00 |
Refinement procedure
Resolution | 19.900 - 1.900 |
R-factor | 0.177 |
Rwork | 0.176 |
R-free | 0.21100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zvv |
RMSD bond length | 0.012 |
RMSD bond angle | 1.289 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.900 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 211799 | |
<I/σ(I)> | 11.74 | 4.49 |
Completeness [%] | 99.4 | 99.1 |
Redundancy | 5.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 296 | PEG 3350 (22.5% (w/v), 100 mM Li2SO4, 100 mM Tris/HCl, VAPOR DIFFUSION, HANGING DROP, temperature 296K, pH 8.00 |