2NZD
Nucleosome core particle containing 145 bp of DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 98 |
Detector technology | IMAGE PLATE |
Collection date | 2006-09-05 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.542 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.617, 109.856, 181.217 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.650 |
R-factor | 0.234 |
Rwork | 0.233 |
R-free | 0.28300 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1kx3 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.450 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.700 | 2.790 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.065 | 0.480 |
Total number of observations | 27467 | |
Number of reflections | 56193 | |
<I/σ(I)> | 20.9 | 2.7 |
Completeness [%] | 91.0 | 63.9 |
Redundancy | 6.3 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 85 mM MnCl2, 60 mM KCl, 20 mM K-Cacodylate, 4 mg/ml NCP over well with 1/2 conc., pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MnCl2 | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | K-Cacodylate | ||
4 | 1 | 1 | HOH | ||
5 | 1 | 2 | MnCl2 | ||
6 | 1 | 2 | KCl | ||
7 | 1 | 2 | K-Cacodylate | ||
8 | 1 | 2 | HOH |