2NYI
Crystal Structure of an Unknown Protein from Galdieria sulphuraria
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97923, 0.96400 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.013, 79.991, 55.530 |
Unit cell angles | 90.00, 111.25, 90.00 |
Refinement procedure
Resolution | 45.620 - 1.800 |
R-factor | 0.178 |
Rwork | 0.176 |
R-free | 0.21400 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.287 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.620 | 45.616 | 1.840 |
High resolution limit [Å] | 1.800 | 4.440 | 1.800 |
Rmerge | 0.071 | 0.051 | 0.419 |
Number of reflections | 39789 | ||
<I/σ(I)> | 11.673 | 2.656 | |
Completeness [%] | 96.3 | 99 | 83.2 |
Redundancy | 7.4 | 7.6 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Protein solution (10 mg/mL protein, 0.050 M Sodium chloride, 0.0003 M TCEP, 0.005 M MES pH 6.0) mixed in a 1:1 ratio with the well solution (24% PEG 8000, 0.04 M Magnesium chloride, 0.2 M Sodium fluoride, 0.10 M PIPES pH 6.5). Cryoprotection: well solution supplemented with up to 20% ethylene glycol, vapor diffusion, hanging drop, temperature 293K |