2NYG

Crystal structure of YokD protein from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-14
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 21
Unit cell lengths	73.020, 132.390, 185.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.040 - 2.600
R-factor	0.257
Rwork	0.257
R-free	0.29600
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.450
High resolution limit [Å]	2.370	2.370
Rmerge	0.073	0.490
Number of reflections	72328
<I/σ(I)>	11.6
Completeness [%]	96.4	82.7
Redundancy	12.1	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	273	25% PEG 3350, 0.2M Lithium sulphate, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 273K
1	VAPOR DIFFUSION, SITTING DROP	5.5	273	25% PEG 3350, 0.2M Lithium sulphate, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 273K