2NY7
HIV-1 gp120 Envelope Glycoprotein Complexed with the Broadly Neutralizing CD4-Binding-Site Antibody b12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-13 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 101.955, 101.955, 298.294 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.450 - 2.300 |
R-factor | 0.19601 |
Rwork | 0.193 |
R-free | 0.25503 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdb entries 1HZH 1gc1 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.852 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.096 | 0.057 | 0.755 |
Number of reflections | 40798 | ||
<I/σ(I)> | 9.8 | ||
Completeness [%] | 97.2 | 98.7 | 77.5 |
Redundancy | 8.8 | 9.7 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |