2NXI
Structural and mechanistic changes along an engineered path from metallo to non-metallo KDO8P synthase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.883, 197.465, 124.874 |
| Unit cell angles | 90.00, 93.84, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fwn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.128 |
| Number of reflections | 123597 |
| <I/σ(I)> | 8.3 |
| Completeness [%] | 76.3 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
