2NX5

Crystal structure of ELS4 TCR bound to HLA-B*3501 presenting EBV peptide EPLPQGQLTAY at 1.7A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Collection date	2005-11-07
Spacegroup name	P 1 21 1
Unit cell lengths	118.479, 118.067, 131.465
Unit cell angles	90.00, 96.04, 90.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.27169
Rwork	0.269
R-free	0.32702
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zsd 2nw2
RMSD bond length	0.006
RMSD bond angle	0.959
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
High resolution limit [Å]	2.700
Number of reflections	87783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	277	0.1M Na Cacodylate (pH6.4), 0.2M CaCl2, 16% PEG 3350, 3% 1,6Hexanediol, vapor diffusion, hanging drop, temperature 277K