2NW4
Crystal Structure of the Rat Androgen Receptor Ligand Binding Domain Complex with BMS-564929
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-04-24 |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.640, 65.620, 70.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.200 - 3.000 |
Rwork | 0.249 |
R-free | 0.29300 |
Structure solution method | MR |
Starting model (for MR) | 1i37 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.146 | 0.456 |
Number of reflections | 5527 | |
<I/σ(I)> | 13 | 3.4 |
Completeness [%] | 99.4 | 98.5 |
Redundancy | 6.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 0.88M NA TARTRATE, 0.1M NA HEPES, VAPOR DIFFUSION, HANGING DROP |