2NVY

RNA Polymerase II form II in 150 mM Mn+2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.17
Spacegroup name	I 2 2 2
Unit cell lengths	118.036, 218.914, 369.549
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.400
R-factor	0.25121
Rwork	0.249
R-free	0.32289
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i50
RMSD bond length	0.012
RMSD bond angle	1.427
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.500
High resolution limit [Å]	3.400	3.400
Number of reflections	64867
<I/σ(I)>	25.6	4.7
Redundancy	5.2	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 50mM dioxane, 10mM DTT, 12% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K