2NV9
The X-ray Crystal Structure of the Paramecium bursaria Chlorella virus arginine decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-11-25 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.98066 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 116.108, 116.874, 269.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.910 - 1.950 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.24200 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.412 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.073 | 0.252 |
| Number of reflections | 235979 | |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 88.7 | 50.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 6% PEG-8000, 100 mM imidazole, 200 mM calcium acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
