2NV5
Crystal structure of a C-terminal phosphatase domain of Rattus norvegicus ortholog of human protein tyrosine phosphatase, receptor type, D (PTPRD)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2006-11-02 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97958 |
Spacegroup name | P 61 |
Unit cell lengths | 140.789, 140.789, 112.431 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.890 - 2.000 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.23700 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.407 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.890 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.126 | 0.837 |
Total number of observations | 149687 | |
Number of reflections | 83626 | |
<I/σ(I)> | 18 | 3.4 |
Completeness [%] | 98.3 | 96.3 |
Redundancy | 16.5 | 12.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 294 | 30% PEG MME 2K, 100mM potassium thiocyanate, pH 7.0, VAPOR DIFFUSION, temperature 294K |