2NUE
Crystal structure of RNase III from Aquifex aeolicus complexed with ds-RNA at 2.9-Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 101.596, 101.657, 67.082 |
Unit cell angles | 90.00, 103.69, 90.00 |
Refinement procedure
Resolution | 29.110 - 2.900 |
R-factor | 0.238 |
Rwork | 0.238 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rc7 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.800 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.072 | 0.160 |
Number of reflections | 13199 | |
<I/σ(I)> | 13.3 | 3.4 |
Completeness [%] | 89.1 | 53.4 |
Redundancy | 2.6 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M NaCl + 16% PEG3350 in 0.1 M Tris-Cl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NaCl | ||
2 | 1 | 1 | PEG3350 | ||
3 | 1 | 1 | Tris-Cl | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | NaCl | ||
6 | 1 | 2 | PEG3350 | ||
7 | 1 | 2 | H2O |