2NQ9
High resolution crystal structure of Escherichia coli endonuclease IV (Endo IV) Y72A mutant bound to damaged DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-10-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 138.500, 59.100, 51.200 |
Unit cell angles | 90.00, 94.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.450 |
Rwork | 0.220 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qtw |
RMSD bond length | 0.004 |
RMSD bond angle | 1.093 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.410 |
Number of reflections | 78368 |
<I/σ(I)> | 18.3 |
Completeness [%] | 98.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 20% MPEG-2000, 0.1 M HEPES pH 7, and 0.4 M sodium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPEG-2000 | ||
2 | 1 | 1 | HEPES | ||
3 | 1 | 1 | sodium acetate | ||
4 | 1 | 2 | MPEG-2000 | ||
5 | 1 | 2 | HEPES | ||
6 | 1 | 2 | sodium acetate |