2NQ3

Crystal structure of the C2 Domain of Human Itchy Homolog E3 Ubiquitin Protein Ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-09-09
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 32 2 1
Unit cell lengths	82.499, 82.499, 65.273
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.100 - 1.800
R-factor	0.16352
Rwork	0.162
R-free	0.19544
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fk9
RMSD bond length	0.018
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	24173
<I/σ(I)>	29.2	3.4
Completeness [%]	100.0	100
Redundancy	10.7	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	The protein at 20 mg/ml was dissolved in 20 mM Tris-HCl, pH 8.0, 0.15 M NaCl, 5% glycerol, 2 mM DTT, and mixed 1:1 with well solution that was 20% PEG3350, 0.1 M bis-Tris, pH 6.0, 0.2 M NH4OAc, 1 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K