2NOX
Crystal structure of tryptophan 2,3-dioxygenase from Ralstonia metallidurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Collection date | 2005-01-01 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 72.544, 132.121, 139.950 |
| Unit cell angles | 66.97, 85.06, 89.89 |
Refinement procedure
| Resolution | 50.120 - 2.400 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yw0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.487 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.074 | 0.974 |
| Number of reflections | 224640 | |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 95.8 | 80.5 |
| Redundancy | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 1.0 M sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
