2NOJ
Crystal structure of Ehp / C3d complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2006-06-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.91840 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.894, 91.025, 122.595 |
| Unit cell angles | 90.00, 89.93, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.29 |
| Rwork | 0.291 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gox |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.337 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.699 |
| Rmerge | 0.189 |
| Number of reflections | 33242 |
| Completeness [%] | 80.8 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 293 | 0.2M LiSO4, 25% PEG 3350, 0.1M Tris-HCl pH 8.2, additive: CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
