2NMV
Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.980 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.410, 95.597, 97.878 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.360 - 2.950 |
R-factor | 0.22706 |
Rwork | 0.224 |
R-free | 0.28074 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2d7d |
RMSD bond length | 0.016 |
RMSD bond angle | 1.713 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.360 | 3.030 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.094 | 0.369 |
Number of reflections | 15109 | |
<I/σ(I)> | 15.9 | 5.2 |
Completeness [%] | 99.1 | 99.3 |
Redundancy | 5.3 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 289 | 18-20% PEG 20000, 0.1M pH 8.5 Tris-Hcl, microbatch, temperature 289K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 20000 | ||
2 | 1 | 1 | Tris-Hcl | ||
3 | 1 | 2 | PEG 20000 |