2NMT
MYRISTOYL-COA:PROTEIN N-MYRISTOYLTRANSFERASE BOUND TO MYRISTOYL-COA AND PEPTIDE ANALOGS
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9668, 0.9789, 0.9792 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 105.400, 105.400, 106.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.29700 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.100 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.3) |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.900 | |
| Rmerge | 0.044 | 0.200 * |
| Total number of observations | 98161 * | |
| Number of reflections | 14314 | |
| Completeness [%] | 91.9 * | 86.8 * |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.25 * | 4 * | pH 6.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | enzyme | 50 (mg/ml) | |
| 10 | 1 | reservoir | glycerol | 21.5 (%(v/v)) | |
| 2 | 1 | 1 | HEPES | 50 (mM) | |
| 3 | 1 | 1 | EDTA | 1 (mM) | |
| 4 | 1 | 1 | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | glycerol | 18 (%(v/v)) | |
| 6 | 1 | drop | protein | 25-30 (mg/ml) | |
| 7 | 1 | reservoir | PEG4000 | 9-11 (%) | |
| 8 | 1 | reservoir | zinc acetate | 50 (mM) | |
| 9 | 1 | reservoir | sodium cacodylate | 100 (mM) |
