2NL8
The origin binding domain of the SV40 large T antigen bound non specifically to a 17 bp palindrome DNA (sites 1 and 3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-05-26 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 43.870, 43.870, 238.308 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.920 - 2.300 |
R-factor | 0.23999 |
Rwork | 0.237 |
R-free | 0.29895 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1IPR |
RMSD bond length | 0.018 |
RMSD bond angle | 2.191 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 11307 | |
<I/σ(I)> | 18.1 | 2.1 |
Completeness [%] | 94.0 | 93 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 20 mM NaAcetate, 20% PEG 3000 + 4% PEG 600, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NaAcetate | ||
2 | 1 | 1 | PEG 3000 | ||
3 | 1 | 1 | PEG 600 | ||
4 | 1 | 2 | NaAcetate | ||
5 | 1 | 2 | PEG 3000 | ||
6 | 1 | 2 | PEG 600 |