2N1F
Structure and assembly of the mouse ASC filament by combined NMR spectroscopy and cryo-electron microscopy
Spectrometer
Experimental method: ELECTRON MICROSCOPY, SOLID-STATE NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE | Bruker Avance | 850 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | NCACX |
| 10 | 1 | 1 | CAN(CO)CA |
| 11 | 1 | 1 | N(CA)CBCX |
| 12 | 1 | 1 | 100msPDSD |
| 2 | 1 | 1 | NCOCX |
| 3 | 1 | 1 | NCACB |
| 4 | 1 | 1 | CANCO |
| 5 | 1 | 1 | CCC |
| 6 | 1 | 1 | NCA |
| 7 | 1 | 1 | NCO |
| 8 | 1 | 1 | 20msDARR |
| 9 | 1 | 1 | N(CO)CACB |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | ambient | 288 |
Conformers
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 10 |
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | TUNGSTEN HAIRPIN |
| Accelerating Voltage (kV) | 300 |
| Detector | DIRECT ELECTRON DE-10 (5k x 4k) |
| Number of Images | 21138 |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4 |
| Software | EMAN,IHRSR,SPIDER |
