2MXS

Solution NMR-structure of the neomycin sensing riboswitch RNA bound to paromomycin

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	DRX	Bruker DRX	600
2	Bruker	AVANCE	Bruker Avance	600
3	Bruker	AVANCE	Bruker Avance	700
4	Bruker	AVANCE	Bruker Avance	800
5	Bruker	AVANCE	Bruker Avance	900
6	Bruker	AVANCE	Bruker Avance	950

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-1H NOESY
10	2	3	3D 1H-13C NOESY-HMQC aromatic
11	2	3	3D HCCH-COSY
12	2	3	3D HCCH-TOCSY
13	2	3	2D 1H-15N lrHSQC
14	2	3	2D DQF-COSY
15	2	3	2D 1H-31P HCP
16	2	3	3D HCCH-TOCSY-E.COSY
17	2	3	2D 13C filter NOESY
18	2	3	2D 13C filter TOCSY
19	2	3	2D quant 31P coupled 1H, 13C HSQC
2	1	2	2D 1H-15N HSQC
20	2	3	2D quant 31P coupled 1H-13C HMQC
3	1	2	2D 1H-13C HNCO
4	1	2	2D 1H-15N H56C56C4N3H
5	1	2	2D 1H-15N HSQC NH2 only
6	2	3	2D 1H-13C HSQC aromatic
7	2	3	2D 1H-13C HSQC aliphatic
8	2	4	2D 1H-13C HSQC aliphatic
9	2	3	3D 1H-13C NOESY-HSQC aliphatic

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.2	ambient	283
2	6.2	ambient	298

Conformers

Conformers Calculated Total Number	100
Conformers Submitted Total Number	20