2M5K
Atomic-resolution structure of a doublet cross-beta amyloid fibril
Spectrometer
Experimental method: ELECTRON MICROSCOPY, SOLID-STATE NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | Bruker | 900 | |
| 2 | 750 | |||
| 3 | 500 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 1D DQ-DRAWS |
| 2 | 1 | 1 | REDOR |
| 3 | 1 | 1 | ZF-TEDOR |
| 4 | 1 | 1 | 2D PDSD |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 2 | ambient |
Conformers
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 1 |
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 250 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 12.7 |
| Software | BKRP,IMAGIC,SPIDER |
