2LFS

Solution structure of the chimeric Af1503 HAMP- EnvZ DHp homodimer; A219F variant

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE		600
2	Bruker	AVANCE III		800
3	Bruker	AVANCE		900

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	2	3D HNCO
10	1	2	2D 12C FILTERED/13C EDITED NOESY
11	1	1	2D 12C/14N FILTERED 1H-1H NOESY
12	1	1	2D 1H-15N TROSY
13	1	1	2D 1H-15N ANTITROSY
2	1	2	3D HNCA
3	1	2	3D CBCA(CO)NH
4	1	2	3D CCH-TOCSY
5	1	1	3D 1H-15N NOESY
6	1	1	3D NNH NOESY
7	1	2	3D 1H-13C NOESY
8	1	2	3D CNH NOESY
9	1	2	3D CCH NOESY

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	7.1	AMBIENT	308

Conformers

Conformers Calculated Total Number	50
Conformers Submitted Total Number	19