2K2U

NMR Structure of the complex between Tfb1 subunit of TFIIH and the activation domain of VP16

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Varian	UNITY	Varian Unity	500
2	Varian	UNITY	Varian Unity	600

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-15N HSQC
10	1	3	2D 1H-15N HSQC
11	1	4	3D HNCO
12	1	4	3D HNCACB
13	1	4	(HB)CBCA(CO)NNH
14	1	4	C(CO)NNH
15	1	3	3D-15N-EDITED-NOESY-HSQC
16	1	4	3D-15N-13C-EDITED-NOESY-HSQC
17	1	4	3D-15N-13C-FILTRED-EDITED-NOESY
18	1	4	2D 1H-13C HSQC
2	1	2	HNCO
3	1	2	HNCACB
4	1	2	(HB)CBCA(CO)NNH
5	1	2	C(CO)NNH
6	1	1	3D-15N-EDITED-NOESY-HSQC
7	1	2	3D-13C-EDITED-HMQC-NOESY
8	1	2	3D-15N-13C-FILTRED-EDITED-NOESY
9	1	2	2D 1H-13C HSQC

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.5	Ambient	298

Conformers

Conformers Calculated Total Number	100
Conformers Submitted Total Number	20