2JLQ

Dengue virus 4 NS3 helicase structure, apo enzyme.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	52.821, 92.614, 102.962
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.960 - 1.670
R-factor	0.21212
Rwork	0.211
R-free	0.23384
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bmf
RMSD bond length	0.002
RMSD bond angle	0.410
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.100	1.800
High resolution limit [Å]	1.700	1.700
Rmerge	0.040	0.760
Number of reflections	59272
<I/σ(I)>	22.4	2.7
Completeness [%]	99.5	95.8
Redundancy	7.5	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1