2JLA
Crystal structure of E.coli MenD, 2-succinyl-5-enolpyruvyl-6-hydroxy- 3-cyclohexadiene-1-carboxylate synthase - SeMet protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-17 |
Detector | ADSC CCD |
Spacegroup name | P 65 |
Unit cell lengths | 95.830, 95.830, 463.170 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 83.050 - 2.810 |
R-factor | 0.172 |
Rwork | 0.169 |
R-free | 0.23300 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.353 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0046) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.000 | 2.880 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.100 | 0.260 |
Number of reflections | 57944 | |
<I/σ(I)> | 20.6 | 6.4 |
Completeness [%] | 99.5 | 96.8 |
Redundancy | 10.3 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 100 MM TRIS-HCL PH 8.5, 22 % PEG 8K, 15 % GLYCEROL, 200 MM NACL |