2JKZ
SACCHAROMYCES CEREVISIAE HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE IN COMPLEX WITH GMP (GUANOSINE 5'- MONOPHOSPHATE) (ORTHORHOMBIC CRYSTAL FORM)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-10 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 118.077, 176.362, 91.109 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.940 - 3.450 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jky |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.640 |
High resolution limit [Å] | 3.450 | 3.450 |
Rmerge | 0.090 | 0.660 |
Number of reflections | 25753 | |
<I/σ(I)> | 10.5 | 1.9 |
Completeness [%] | 99.4 | 100 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | 1.6 M AMMONIUM SULPHATE, 4% PEG 400, 0.2 M NA-K TARTRATE, 50 MM NA CITRATE, 50 MM MES, PH 5.8, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |