2JKZ

SACCHAROMYCES CEREVISIAE HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE IN COMPLEX WITH GMP (GUANOSINE 5'- MONOPHOSPHATE) (ORTHORHOMBIC CRYSTAL FORM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-10
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	118.077, 176.362, 91.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.940 - 3.450
R-factor	0.221
Rwork	0.221
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jky
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.640
High resolution limit [Å]	3.450	3.450
Rmerge	0.090	0.660
Number of reflections	25753
<I/σ(I)>	10.5	1.9
Completeness [%]	99.4	100
Redundancy	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	293	1.6 M AMMONIUM SULPHATE, 4% PEG 400, 0.2 M NA-K TARTRATE, 50 MM NA CITRATE, 50 MM MES, PH 5.8, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K