2JK4
Structure of the human voltage-dependent anion channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 77.990, 77.990, 167.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 4.100 |
| R-factor | 0.3314 |
| Rwork | 0.325 |
| R-free | 0.38730 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXD (SOLOMON SHARP) |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 4.300 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.100 | 0.540 |
| Number of reflections | 4887 | |
| <I/σ(I)> | 16.4 | 3.5 |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 16.8 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M MAGNESIUM CHLORIDE HEXAHYDRATE, 0.1 M HEPES SODIUM PH 7.5, 30% V/V POLYETHYLENE GLYCOL 400 |
