2JJ6
Crystal structure of the putative carbohydrate recognition domain of the human galectin-related protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 173 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 78.391, 78.391, 89.883 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.25890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bkz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.044 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.390 |
| Number of reflections | 21932 | |
| <I/σ(I)> | 24.4 | 4.6 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | TRIS/HCL, PH8.0, 14% PEG6000 |
