2JIX

Crystal structure of ABT-007 FAB fragment with the soluble domain of EPO receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	117.954, 156.171, 164.205
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	113.230 - 3.200
R-factor	0.26
Rwork	0.257
R-free	0.32300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	2.608
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.080	0.510
Number of reflections	50772
<I/σ(I)>	15.4	2
Completeness [%]	97.4	81.7
Redundancy	6.7	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1