2JGT
Low resolution structure of SPT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 93.866, 109.537, 187.031 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.990 - 3.000 |
R-factor | 0.251 |
Rwork | 0.250 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fc4 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.316 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.070 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.080 | 0.490 |
Number of reflections | 19635 | |
<I/σ(I)> | 21 | 5 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 7.8 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 10% PEG 8K, 0.17 M MAGNESIUM CHLORIDE, 0.1 M TRIS PH 6.5 |