2JG7
Crystal structure of Seabream Antiquitin and Elucidation of its substrate specificity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS |
Synchrotron site | PAL/PLS |
Temperature [K] | 100 |
Spacegroup name | P 1 |
Unit cell lengths | 88.160, 111.020, 113.640 |
Unit cell angles | 90.50, 98.10, 111.59 |
Refinement procedure
Resolution | 30.190 - 2.830 |
R-factor | 0.211 |
Rwork | 0.210 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bpw |
RMSD bond length | 0.008 |
RMSD bond angle | 1.062 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.200 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.120 | 0.300 |
Number of reflections | 86382 | |
<I/σ(I)> | 7.3 | 2.1 |
Completeness [%] | 93.0 | 85 |
Redundancy | 2.9 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |