Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.727, 130.165, 126.726 |
| Unit cell angles | 90.00, 93.61, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.650 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ewo |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.507 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.360 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.080 | 0.440 |
| Number of reflections | 417377 | |
| <I/σ(I)> | 8.4 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN WAS CRYSTALLIZED FROM 1.5M SODIUM CHLORIDE, 1.6M AMMONIUM SULFATE, 0.1M HEPES PH 7.5, 5MM AGMATINE |
