2JCN
The crystal structure of BAK1 - a mitochondrial apoptosis regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 62.770, 62.770, 138.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.690 - 1.800 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.23200 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.426 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.440 |
| Number of reflections | 313450 | |
| <I/σ(I)> | 38 | 8.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 20.17 | 20.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 22% PEG3350, 20% GLYCEROL, 0.2M SODIUM SULPHATE, 2MM TCEP, 0.3M NACL, 20 MM HEPES PH7.5, pH 7.50 |
