2JCK
Crystal structure of alpha-1,3 Galactosyltransferase (R365K) in complex with UDP and 2 manganese ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-25 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 122.824, 68.251, 44.732 |
Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
Resolution | 24.840 - 1.800 |
R-factor | 0.182 |
Rwork | 0.182 |
R-free | 0.19200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k4v |
RMSD bond length | 0.007 |
RMSD bond angle | 1.390 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.100 | 0.210 |
Number of reflections | 34457 | |
<I/σ(I)> | 9 | 5.14 |
Completeness [%] | 91.7 | 93.6 |
Redundancy | 4.5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 0.1M TRIS-HCL, PH 8.0, 10% PEG 6000, AND 5% MPD |