2JC5

Apurinic Apyrimidinic (AP) endonuclease (NApe) from Neisseria Meningitidis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-21
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	38.156, 79.511, 41.045
Unit cell angles	90.00, 107.22, 90.00

Refinement procedure

Resolution	29.530 - 1.500
R-factor	0.123
Rwork	0.120
R-free	0.15900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bix
RMSD bond length	0.010
RMSD bond angle	1.330
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.060	0.270
Number of reflections	35560
<I/σ(I)>	18.3	4.5
Completeness [%]	94.6	86.3
Redundancy	3.5	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9		24 WELL LINBRO PLATES USED FOR HANGING DROP EXPERIMENTS WITH 0.5 UL PROTEIN AND 0.5 UL WELL SOLUTION FOR THE DROPS. PROTEIN CONCENTRATION 20 MG/ML, IN 10 MM TRIS, PH 7.0 AND 100 MM NACL. THE WELL SOLUTION CONTAINED 20% PEG 20000, 0.1 M BICINE PH 9.0, 2% DIOXANE.