2JC3
Structure of O-Acetylserine Sulfhydrylase B from Salmonella Typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 103.052, 106.948, 112.107 |
| Unit cell angles | 95.59, 90.06, 117.61 |
Refinement procedure
| Resolution | 19.900 - 2.300 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oas |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 0.190 |
| Number of reflections | 179465 | |
| <I/σ(I)> | 5 | 1.5 |
| Completeness [%] | 96.2 | 91.2 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | PRECIPITANT: 1M NA-K TARTARATE BUFFER: 0.1 M MES, PH 6.0 PROTEIN CONCENTRATION: 23 MG/ML DROP VOLUME: 4 UL (TOTAL) |
