2JC0
CRYSTAL STRUCTURE OF HEPATITIS C VIRUS POLYMERASE IN COMPLEX WITH INHIBITOR SB655264
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.740, 105.893, 126.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.24800 |
| Structure solution method | OTHER |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.705 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.3.0006) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.200 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.140 |
| Number of reflections | 75239 |
| Completeness [%] | 96.5 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG4K, 100MM CITRATE BUFFER, PH5.0, 5MM DTT, 10% GLYCEROL |
