2JBJ
membrane-bound glutamate carboxypeptidase II (GCPII) in complex with 2-PMPA (2-phosphonoMethyl-pentanedioic acid)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 100.755, 130.982, 159.611 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 101.020 - 2.190 |
R-factor | 0.193 |
Rwork | 0.192 |
R-free | 0.23800 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.023 |
RMSD bond angle | 2.077 |
Data reduction software | HKL |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.090 |
Number of reflections | 52532 |
<I/σ(I)> | 13.1 |
Completeness [%] | 95.9 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20 MM HEPES PH 7.25, 200 MM NACL, 5% (W/V) PEG 400 AND 15% (W/V) PEG 1500. |