2JAS
Structure of deoxyadenosine kinase from M.mycoides with bound dATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-25 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.845, 100.850, 109.486 |
| Unit cell angles | 90.00, 124.58, 90.00 |
Refinement procedure
| Resolution | 47.000 - 2.700 |
| R-factor | 0.235 |
| Rwork | 0.233 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p5z |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.143 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.100 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 46941 | |
| <I/σ(I)> | 15.6 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 24% PEG 3350 0.2 M KSCN, pH 7.00 |
