2JAN
TYROSYL-TRNA SYNTHETASE FROM MYCOBACTERIUM TUBERCULOSIS IN UNLIGANDED STATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.262, 78.440, 81.633 |
| Unit cell angles | 74.42, 83.19, 82.95 |
Refinement procedure
| Resolution | 19.890 - 2.900 |
| R-factor | 0.204 |
| Rwork | 0.201 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ts1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.104 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.890 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.120 | 0.630 |
| Number of reflections | 36581 | |
| <I/σ(I)> | 8.63 | 1.86 |
| Completeness [%] | 98.4 | 97.6 |
| Redundancy | 2.92 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% (W/V) PEG 6000, 1 M LICL, 0.1 M HEPES AT PH 7.0 |
