2JAG
L1-intermediate of halorhodopsin T203V
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | H 3 2 |
Unit cell lengths | 95.042, 95.042, 157.674 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.930 |
R-factor | 0.2373 |
Rwork | 0.237 |
R-free | 0.26080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e12 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.400 |
Data reduction software | XDS |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 1.800 | |
Rmerge | 0.120 | 0.450 |
Number of reflections | 23209 | |
<I/σ(I)> | 17.1 | 3.2 |
Completeness [%] | 87.8 | 73.1 |
Redundancy | 8.5 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |