2J9L
Cytoplasmic Domain of the Human Chloride Transporter ClC-5 in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 122.328, 148.101, 79.883 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.264 |
| Rwork | 0.263 |
| R-free | 0.29500 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.887 |
| Data reduction software | DENZO |
| Data scaling software | TRUNCATE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 60291 | |
| <I/σ(I)> | 18.1 | 2.23 |
| Completeness [%] | 95.3 | 68.5 |
| Redundancy | 5.2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM (NH4)2SO4, 10 MM TRIS HCL (PH 7.4), 18% PEG 4000 |
